Protein purification : principles, high resolution methods, and applications / edited by Jan-Christer Janson.Material type: TextLanguage: English Series: Methods of biochemical analysisPublication details: Hoboken, N.J. : John Wiley & Sons, c2011. Edition: 3rd edDescription: xiv, 517 p. : ill. (some col.) ; 29 cmISBN: 9780471746614 (cloth); 0471746614 (cloth)Subject(s): Proteins -- Purification | Chromatographic analysis | Electrophoresis | Proteins -- isolation & purification | Chromatography -- methods | Electrophoresis -- methodsDDC classification: 572.6 LOC classification: QP551 | .P69754 2011NLM classification: QU 55Online resources: WorldCat details
|Item type||Current library||Collection||Call number||Copy number||Status||Date due||Barcode||Item holds|
|Text||EWU Library Reserve Section||Non-fiction||572.6 PRO 2011 (Browse shelf(Opens below))||C-1||Not For Loan||27141|
Includes bibliographical references and index.
TOC Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson -- Introduction to chromatography / J.-C. Janson and J.Å. Jönsson -- Gel filtration: Size exclusion chromatography / L. Hagel -- Ion exchange chromatography / E. Karlsson and I. Hirsh -- High-resolution reversed-phase chromatography of proteins / S.W. Pettersson -- Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew -- Immobilized metal ion affinity chromatography / L. Kågedal -- Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson -- Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson -- Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann -- Affinity ligands from biological combinatorial libraries / P.-Å. Nygren -- Membrane separations / J.K. Walter ... [et al.] -- Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu -- Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine -- Electrophoresis in gels / R. Westermeier -- Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti -- Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg -- Protein elution and blotting techniques / R. Westermeier -- Capillary electrophoretic separations / W. Thormann -- High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.
The authoritative guide on protein purification-now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research.