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Animal cell culture : essential methods / editor, John M. Davis.

Contributor(s): Davis, John, 1952-.
Material type: TextTextPublisher: Hoboken, NJ : John Wiley & Sons Inc., 2011Description: xxvii, 346 p. : ill. ; 25 cm.ISBN: 9780470666586 (pbk.).Subject(s): Cell culture -- Technique | Tissue culture -- TechniqueDDC classification: 571.6381 ANI Online resources: OCLC | Ebook Fulltext
Contents:
The cell culture laboratory / Sue Clarke and Janette Dillon -- Sterilization / Peter L. Roberts -- Microscopy of living cells / Colin Gray and Daniel Zicha -- Basic techniques and media, the maintenance of cell lines, and safety / John M. Davis -- Development and optimization of serum- and protein-free culture media / Stephen F. Gorfien and David W. Jayme -- Cryopreservation and banking of cell lines / Glyn N. Stacey, Ross Hawkins and Roland A. Fleck -- Primary culture of specific cell types and the establishment of cell lines / Kee Woei Ng, ... [et al.] -- Cloning / John Clarke, Alison Porter and John M. Davis -- The quality control of animal cell lines and the prevention, detection and cure of contamination / Peter Thraves and Cathy Rowe -- Systems for cell culture scale-up / Jennifer Halsall and John M. Davis -- Good laboratory practice in the cell culture laboratory / Barbara Orton
Summary: This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench.
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Includes bibliographical references and index.

The cell culture laboratory / Sue Clarke and Janette Dillon --
Sterilization / Peter L. Roberts --
Microscopy of living cells / Colin Gray and Daniel Zicha --
Basic techniques and media, the maintenance of cell lines, and safety / John M. Davis --
Development and optimization of serum- and protein-free culture media / Stephen F. Gorfien and David W. Jayme --
Cryopreservation and banking of cell lines / Glyn N. Stacey, Ross Hawkins and Roland A. Fleck --
Primary culture of specific cell types and the establishment of cell lines / Kee Woei Ng, ... [et al.] --
Cloning / John Clarke, Alison Porter and John M. Davis --
The quality control of animal cell lines and the prevention, detection and cure of contamination / Peter Thraves and Cathy Rowe --
Systems for cell culture scale-up / Jennifer Halsall and John M. Davis --
Good laboratory practice in the cell culture laboratory / Barbara Orton

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench.

Pharmacy

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