Culture of animal cells : a manual of basic technique and specialized applications / R. Ian Freshney, reviewing editors, Amanda Capes-Davis, Carl Gregory, Stefan Przyborski.

Includes bibliographical references and index.

List of Figures, xix List of Color Plates, xxiii List of Tables, xxv List of Protocols, xxvii List of Minireviews, xxix Preface and Acknlowledgments, xxxi Abbreviations, xxxiii About the Companion Website xxxviii 1. Introduction, 1 2. Biology of Cultured Cells, 19 3. Laboratory Design and Layout, 37 4. Equipment and Materials, 51 5. Aseptic Technique, 73 6. Safety, Bioethics, and Validation, 89 7. Culture Vessels and Substrates, 111 8. Defined Media and Supplements, 125 9. Serum-Free Media, 149 10. Preparation and Sterilization, 173 10. Supplementary Material* 11. Primary Culture, 207 11. Supplementary Material* 12. Subculture and Cell Lines, 235 13. Authentication and Validation, 259 14. Microbial Contamination, 289 15. Cryopreservation and Banking, 307 16. Cloning and Selection, 327 16. Supplementary Material* 17. Cell Sorting, 347 18. Cell Line Characterization, 359 19. Differentiation, 383 20. Three ]Dimensional Culture 401 21. Scale ]up and Automation, 427 22. Senescence, Immortalization, and Transformation, 455 23. Quantitation, 483 23 Supplementary Material* 24. Cytotoxicity, 513 24. Supplementary Material* 25. Culture of Specific Cell Types, 531 25. Supplementary Material* 26. Stem Cells, 571 26. Supplementary Material* 27. Training Programs, 591 28. Problem Solving 627 29. In Conclusion, 651 Supplementary Material* Appendix III: Sources of Equipment and Material Appendix IV: Suppliers and Other Resource

GEB

Abdul Latif